The standard protocol recommends to add 400 uL QGel™ Buffer A and 100 uL cell suspension for a total of 500 uL resuspension volume.

The cell suspension volume can be lowered but QGel™ Buffer A volume has to be increased accordingly in order to keep the 500 uL total resuspension volume.

Similarly, if the volume of the cell suspension is increased, the QGel™ Buffer A volume has to be decreased accordingly (to keep the 500 uL total resuspension volume). Please take note that decreasing Buffer A volume may alter the gelation conditions of the QGel™ matrix. Consequently, the final gel characteristics may be different than those guaranteed by QGel™.