No, it is not possible to use half vial and freeze the remaining. Each QGel™ MT 3D Matrix vial can be used only once because hydrogel formation starts as soon as the powder is resuspended into the QGel™ Buffer. A freezing and thawing process will alter the properties of the gel.

Note that 500 uL of gel can be made from one QGel™ MT 3D Matrix vial and this is usually the minimum material required to perform an experiment. Indeed, one experiment involving different conditions with several time points and analysis including also replicates may require easily 500 uL gel.

Besides QGel™ has prepared a table to help you design your experiment with QGel™ products. It allows you to determine quickly the number of QGel™ vials and QGel™ Disc Casters you need for your experiment. For more information and an example how to use this table, please see the question How to design my experimental plan with QGel MT 3D Matrix?.