The cell density needs to be optimized for each cell type and experimental conditions. Indeed, cell density is a critical factor for cell survival and proliferation. You can start based on the cell density used in your previous 3D studies with these cells if good results were obtained. We consider 1×10⁵ cells/mL gel as low cell density and 10×10⁶ cells/mL gel as very high cell density. No cell loading limit has been reported for the moment.

For more information, please refer to previous publications to get an idea of the different cell density used with QGel™ matrices in the past (see table below).