Help Center/Cell Encapsulation & Gel Handling

How do I encapsulate cells and make 3D gel discs?

QGel Support
posted this on June 04, 2010 14:27

A good video to watch is definitely How to encapsulate cells in QGel.

You can also find information directly in p.08-09 of the QGel Product Information Brochure. Or, even simpler, you can download a 1-page QuickGuide to Cast Gels (images below) and tape it directly in your lab book.

Below are some tricks how to organize yourself before starting an experiment with QGel :

1)       Prepare the material to make gels (e.g. disc casters, spatula, 24-well plates) and allow QGel™ products (powder and buffer) required for the experiment to reach room temperature.

2)     Immerse gel caster(s) in 70% ethanol for sterilization (10-20min) and allow drying in the hood.

 What is the number of gel casters required? Example: If you make 30uL gel discs or drops you can fit up to 8-10 per gel caster.  The number decreases with increasing volume of the drops. With one vial you can make up to 15-16 discs of 30uL. 

3)       While gel caster are drying, prepare the cell suspension to be incorporated. Then, label and add medium to the wells of the 24-well plate(s) according to the number of gel discs that will be casted (store the well plates in the incubator during cell encapsulation experiments). 

4)       Are the buffer and the gel vials at room temperature? Are the gel casters dry? Are the cell suspensions ready at the right concentration? Is the cell culture medium added to the wells where the gels with cells will be placed later on? If "yes" to all of the above, then you can start your encapsulation experiment.

Note regarding harvesting: After 45 minutes incubation at 37°C the gel discs in the caster can be harvested. The video  How to encapsulate cells in QGel describes well the key steps of the harvesting process. Useful tricks to avoid gel sticking can be found on this FAQ.

Note regarding your experimental plan: The FAQ What to do in my first experiment with QGel matrix? will give you good advice about how to design your very first experiment with QGel MT 3D Matrix.

 

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